Fig. 3
From: Establishment of BmoR-based biosensor to screen isobutanol overproducer
Optimization of copy number of the plasmid for BmoR-Pbmo device construction via blue–white screening experiments. The BmoR-Pbmo device in the strain YHS1 (BW25113 (F′) harboring pSA69 and pYH4) was constructed in a high-copy-number plasmid. The BmoR-Pbmo device in the strain YHS2 (BW25113 (F′) harboring pSA69, pSA65 and pYH5) was constructed in a medium-copy-number plasmid. The BmoR-Pbmo device in the strain YHS3 (BW25113 (F′) harboring pSA69, pSA65 and pYH3) was constructed in a low-copy-number plasmid