Fig. 1
From: Establishment of BmoR-based biosensor to screen isobutanol overproducer
The pathway for isobutanol production and mechanism of BmoR-based biosensor response to isobutanol via detection of GFP fluorescence. AlsS, acetolactate synthase; IlvC, acetohydroxy acid isomeroreductase; IlvD, dihydroxy-acid dehydratase; IlvE, branched-chain-amino-acid transaminase; Kivd, ketoisovalerate decarboxylase; AdhA, alcohol dehydrogenase. PbmoR, a constitutive promoter to regulate the BmoR expression. Promoter Pbmo drove the GFP expression and was regulated by BmoR protein. Isobutanol generated from glucose bound to BmoR and then activated Eσ54 closed complex to induce the transcription initiation of Pbmo