Table 1 Bacterial strains and plasmids used in this study

Strains

 Escherichia coli DH5α

Host strain for cloning

NEB

 Escherichia coli BL21

Heterologous expression strain

NEB

 Serratia plymuthica WS3236

Native producer of sodorifen

ZIEL Institute Culture Collection

Plasmids

 pET28b-ptetO-gfpv2

(6029 bp)

Tetracycline inducible expression plasmid, ColE1, Kan^R, gfp reporter gene downstream of promotor

This study

 pET28b-ptetO::sod_gfpv2

(11,124 bp)

pET28b-ptetO_gfpv2 with sodABCD cloned as single fragment between Ptet_O and gfp

This study