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Table 1 Bacterial strains and plasmids used in this study

From: Direct pathway cloning of the sodorifen biosynthetic gene cluster and recombinant generation of its product in E. coli

  Description Reference or source
Strains
 Escherichia coli DH5α Host strain for cloning NEB
 Escherichia coli BL21 Heterologous expression strain NEB
 Serratia plymuthica WS3236 Native producer of sodorifen ZIEL Institute Culture Collection
Plasmids
 pET28b-ptetO-gfpv2
(6029 bp)
Tetracycline inducible expression plasmid, ColE1, KanR, gfp reporter gene downstream of promotor This study
 pET28b-ptetO::sod_gfpv2
(11,124 bp)
pET28b-ptetO_gfpv2 with sodABCD cloned as single fragment between PtetO and gfp This study