Table 2 Primers
From: Microbial production of lipid-protein vesicles using enveloped bacteriophage phi6
Primer | Sequence | Tm, °C* | Plasmid |
|---|---|---|---|
P9_5′_NcoI | 5′-TACAGTCCATGGCTATGCCATTTCCTCTGGTAAAG-3′ | 62.2 | pDS4 |
P9_3′_HindIII | 5′-TAGCACAAGCTTAGGCCAGAAAAGGGATG-3′ | 62.0 | pDS4 |
P12_5′_NcoI | 5′-ATACCATGGTTATCGGTCTCCTGAAG-3′ | 62.3 | pOL2, pOL5 |
P12_3′_EcoRI | 5′-TTAGAATTCATTACGGAACATCCTTACG-3′ | 60.0 | pOL2 |
P9_3′_HindIII_R | 5′-GCGCAAGCTTAGGCCAGAAAAGGGATGTTG-3′ | 66.2 | pOL5 |