Fig. 1

Thin sections of E. coli cells expressing P9 and P12. E. coli BL21(DE3) cells expressing phi6 proteins P9 and P12 were fixed with glutaraldehyde, embedded in Epon, thin sectioned, and analyzed by transmission electron microscopy (TEM). Representative TEM images of cells expressing the major envelope protein P9 from plasmid pDS4 (a), P9 and the non-structural protein P12 from plasmids pDS4 and pOL2, respectively (b), and control cells containing vector plasmid pCDF-1b (c). Each scale bar represents 1 µm. The arrows in a and b point to structures specifically identified in cells expressing P9