Production of l-alanyl-l-glutamine by immobilized Pichia pastoris GS115 expressing α-amino acid ester acyltransferase

Table 3 Effects of various additives on the activity of GPAp

Additive (10 mM)	Relative enzymatic activity (%)
None	100.00 ± 0.00
K⁺	99.82 ± 0.63
Na⁺	101.52 ± 1.34
Ca²⁺	99.20 ± 1.06
Mg²⁺	101.44 ± 0.72
Zn²⁺	104.63 ± 3.04
Fe²⁺	108.36 ± 1.83
Fe³⁺	91.23 ± 4.39
Cysteine	116.50 ± 1.48
EDTA	107.13 ± 2.81
PMSF	112.59 ± 0.71

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