Strain or plasmid | Description | Source |
---|---|---|
Original strains | ||
S. siyangensis SY1 | Template of alpha-amino acid ester acyl transferase gene (SsAET) | CGMCC (1.6855) |
E. coli DH5α | Genetic manipulation | Solarbio (C1100) |
E. coli TOP10 | Genetic manipulation | Solarbio (C1210) |
P. pastoris GS115 | Methylotrophic yeast, capable of metabolizing methanol as individual sole carbon source; a mutation in the histidinol dehydrogenase gene (HIS4); histidine auxotroph | Invitrogen (C18100) |
Recombinant strains | ||
TOP10-pUC57-SsAETp | pUC57-SsAETp plasmid in TOP10; ampicillin-resistant | This study |
DH5α-pPIC9-SsAETp | pPIC9-SsAETp plasmid in DH5α; ampicillin-resistant | This study |
DH5α-pPIC9-SsAET | pPIC9-SsAET plasmid in DH5α; ampicillin-resistant | This study |
GS115-pPIC9-SsAETp (GPAp) | pPIC9-SsAETp plasmid in P. pastoris GS115; His+ MutS | This study |
GS115-pPIC9-SsAET (GPA) | pPIC9-SsAET plasmid in P. pastoris GS115; His+ MutS | This study |
Plasmids | ||
pPIC9 | Empty vector; P. pastoris HIS4 vector for methanol-inducible expression of a secreted protein; ampicillin-resistant | Invitrogen (K1710-01) |
pUC57-SsAETp | pUC57 vector harboring SsAETp gene which codon-optimized SsAET gene according to codon bias of P. pastoris; ampicillin-resistant | This study |
pPIC9-SsAETp | pPIC9 vector harboring SsAETp gene; ampicillin-resistant | This study |
pPIC9-SsAET | pPIC9 vector harboring SsAET gene; ampicillin-resistant | This study |