Skip to main content

Table 1 Descriptions of strains and plasmids used in this study

From: Production of l-alanyl-l-glutamine by immobilized Pichia pastoris GS115 expressing α-amino acid ester acyltransferase

Strain or plasmid

Description

Source

Original strains

 S. siyangensis SY1

Template of alpha-amino acid ester acyl transferase gene (SsAET)

CGMCC (1.6855)

 E. coli DH5α

Genetic manipulation

Solarbio (C1100)

 E. coli TOP10

Genetic manipulation

Solarbio (C1210)

 P. pastoris GS115

Methylotrophic yeast, capable of metabolizing methanol as individual sole carbon source; a mutation in the histidinol dehydrogenase gene (HIS4); histidine auxotroph

Invitrogen (C18100)

Recombinant strains

 TOP10-pUC57-SsAETp

pUC57-SsAETp plasmid in TOP10; ampicillin-resistant

This study

 DH5α-pPIC9-SsAETp

pPIC9-SsAETp plasmid in DH5α; ampicillin-resistant

This study

 DH5α-pPIC9-SsAET

pPIC9-SsAET plasmid in DH5α; ampicillin-resistant

This study

 GS115-pPIC9-SsAETp (GPAp)

pPIC9-SsAETp plasmid in P. pastoris GS115; His+ MutS

This study

 GS115-pPIC9-SsAET (GPA)

pPIC9-SsAET plasmid in P. pastoris GS115; His+ MutS

This study

Plasmids

 pPIC9

Empty vector; P. pastoris HIS4 vector for methanol-inducible expression of a secreted protein; ampicillin-resistant

Invitrogen (K1710-01)

 pUC57-SsAETp

pUC57 vector harboring SsAETp gene which codon-optimized SsAET gene according to codon bias of P. pastoris; ampicillin-resistant

This study

 pPIC9-SsAETp

pPIC9 vector harboring SsAETp gene; ampicillin-resistant

This study

 pPIC9-SsAET

pPIC9 vector harboring SsAET gene; ampicillin-resistant

This study