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Fig. 6 | Microbial Cell Factories

Fig. 6

From: Production of l-alanyl-l-glutamine by immobilized Pichia pastoris GS115 expressing α-amino acid ester acyltransferase

Fig. 6

Application of immobilized GPAp by recycling to maintain the stability of Ala-Gln production. a The catalytic activities of GPAp were detected daily by HPLC during the 5 days which GPAp was stored at the optimal reaction pH and temperature (8.5 at 28 °C). b The catalytic activities were detected and compared among free GPAp with one unit of OD600, immobilized GPAp with one unit of OD600 and immobilized GPAp with double OD600. c The reusability of immobilized GPAp was detected at optimum conditions for recycling 10 times

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