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Table 3 Results of agitation fermentation coupled with static process

From: Large-scale production of tauroursodeoxycholic acid products through fermentation optimization of engineered Escherichia coli cell factory

No. Time agitation process-static process Tube volume (mL) TUDCA (g/L) T-7-KLCA (g/L) TCDCA (g/L) Ratio of TUDCA to TCDCA TUDCA conversion efficiency (%)
1 0 h–0 h 0.00 0.00 1.10 0.00 0.00
2 3.5 h–0 h 0.04 0.00 1.06 0.04 3.55
3 6.5 h–0 h 0.07 0.01 1.00 0.07 6.05
4 16.5 h–0 h 0.18 0.04 0.89 0.20 15.94
5 0 h–16.5 h 2 0.15 0.02 0.93 0.16 13.23
6 15 0.27 0.04 0.79 0.34 24.24
7 50 0.26 0.01 0.83 0.31 23.30
8 0 h–22 h 2 0.19 0.05 0.86 0.22 17.22
9 15 0.30 0.03 0.78 0.40 27.73
10 50 0.28 0.06 0.76 0.36 25.21
11 0 h–48 h 2 0.19 0.05 0.86 0.22 17.01
12 15 0.30 0.06 0.74 0.41 27.22
13 50 0.21 0.16 0.73 0.28 18.92
14 3.5 h–13 h 2 0.54 0.03 0.52 1.05 49.58
15 15 0.56 0.04 0.50 1.11 50.76
16 50 0.58 0.04 0.48 1.20 52.70
17 3.5 h–18.5 h 2 0.54 0.06 0.49 1.10 49.49
18 15 0.53 0.07 0.50 1.05 47.94
19 50 0.52 0.07 0.51 1.02 47.10
20 3.5 h–44.5 h 2 0.50 0.09 0.51 0.98 45.52
21 15 0.51 0.09 0.50 1.02 46.42
22 50 0.51 0.10 0.49 1.05 46.60
23 6.5 h–10 h 2 0.52 0.07 0.51 1.02 47.41
24 15 0.51 0.06 0.52 0.98 46.74
25 50 0.52 0.06 0.52 1.00 47.20
26 6.5 h–15.5 h 2 0.42 0.07 0.61 0.69 38.25
27 15 0.41 0.08 0.61 0.67 37.36
28 50 0.43 0.06 0.60 0.71 39.25
29 6.5 h–41.5 h 2 0.50 0.09 0.51 0.96 45.19
30 15 0.49 0.09 0.52 0.94 44.38
31 50 0.51 0.09 0.50 1.03 46.66
32 16.5 h–5.5 h 2 0.19 0.06 0.85 0.22 17.25
33 15 0.20 0.07 0.83 0.24 17.83
34 50 0.24 0.09 0.76 0.32 22.28
35 16.5 h–31.5 h 2 0.22 0.09 0.80 0.27 19.62
36 15 0.32 0.09 0.69 0.47 29.21
37 50 0.32 0.09 0.69 0.46 28.94
  1. These samples were directly taken from fermenters without static process. TUDCA conversion efficiency above 45.00% was highlighted in italics