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Table 1 Experimental designs and the results of the Box–Behnken Design

From: Large-scale production of tauroursodeoxycholic acid products through fermentation optimization of engineered Escherichia coli cell factory

Std Run Factors Responses
A-glucose
(g/L)
B-glycerol
(mL/L)
C-tryptone
(g/L)
D-yeast extract
(g/L)
OD600 Conversion efficiency (%)
22 1 10 30 10 0 8.36 1.41
14 2 10 30 0 10 10.25 0.17
9 3 0 15 10 0 4.37 12.54
5 4 10 15 0 0 5.64 1.37
23 5 10 0 10 20 10.91 1.11
8 6 10 15 20 20 10.91 3.26
2 7 20 0 10 10 12.04 0.19
28 8 10 15 10 10 11.44 0.00
4 9 20 30 10 10 10.43 0.03
13 10 10 0 0 10 10.81 0.58
29 11 10 15 10 10 12.50 0.00
26 12 10 15 10 10 9.74 0.00
11 13 0 15 10 20 6.25 22.96
12 14 20 15 10 20 13.29 0.45
1 15 0 0 10 10 7.28 3.13
21 16 10 0 10 0 9.51 0.03
25 17 10 15 10 10 8.65 0.01
16 18 10 30 20 10 12.66 0.31
3 19 0 30 10 10 6.61 13.42
27 20 10 15 10 10 10.62 0.00
10 21 20 15 10 0 8.76 0.02
17 22 0 15 0 10 8.50 20.25
19 23 0 15 20 10 6.05 13.58
18 24 20 15 0 10 9.54 0.11
7 25 10 15 0 20 10.21 0.29
15 26 10 0 20 10 10.92 1.02
6 27 10 15 20 0 8.40 0.55
20 28 20 15 20 10 11.76 0.27
24 29 10 30 10 20 10.64 0.38
  1. The highest responses of OD600 and conversion efficiency were highlighted in italics