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Table 1 Bacterial strains and plasmids used in this study

From: A rapid and versatile tool for genomic engineering in Lactococcus lactis

Strain or plasmid Relevant features Source or references
Strains
 E. coli DH5α Subcloning host Our lab
 E. coli XL1-Blue Subcloning host Our lab
 L. lactis NZ9000 L. lactis MG1363 pepN::nisRK; commonly used host for NICE system [43]
 L. lactis IL1403 The source of putative recombinase pi12 [27]
 E. faecalis ATCC14506 The source of recombinase RecT Our lab
 Lb. plantarum WCFS1 The source of recombinase Lp_0641 [4]
 Lb. casei BL23 The source of recombinase LCABL_13050 [5]
 Lb. delbrueckii SDMCC050201 The source of putative recombinase phiJB_00020 [26]
Plasmids
 pLeiss:Sec:Nuc L. lactis and E. coli shuttle cloning vector which contains PnisA promoter used for nisin controlled gene expression; Cmr [44]
 pTRKL2 L. lactis and E. coli shuttle cloning vector which exists at low copy number in L. lactis; Ermr [45]
 pTRKH2 L. lactis and E. coli shuttle cloning vector which exists at high copy number in L. lactis; Ermr [45]
 pCas9 Low copy number vector, expression of Cas9 nuclease, tracrRNA and crRNA in E. coli; Cmr [23]
 pKD46 λ Red recombinase expression vector; the source of recombinase Beta; Ampr [46]
 pTLCas9 Cas9, tracrRNA and crRNA cloning in pTRKL2; Ermr This work
 pTHCas9 Cas9, tracrRNA and crRNA cloning in pTRKH2; Ermr This work
 pTLCas9upp pTLCas9 with a 20 bp spacer targeting the upp gene of L. lactis NZ9000; Ermr This work
 pTLCas9galk1 pTLCas9 with a 20 bp spacer targeting the galK gene of L. lactis NZ9000; Ermr This work
 pTLCas9hemN1 pTLCas9 with a 20 bp spacer targeting the hemN gene of L. lactis NZ9000; Ermr This work
 pTLCas9recA1 pTLCas9 with a 20 bp spacer targeting the recA gene of L. lactis NZ9000; Ermr This work
 pTLCas9noxD1 pTLCas9 with a 20 bp spacer targeting the noxD gene of L. lactis NZ9000; Ermr This work
 pTHCas9galk1 pTHCas9 with a 20 bp spacer targeting the galK gene of L. lactis NZ9000; Ermr This work
 pTHCas9hemN1 pTHCas9 with a 20 bp spacer targeting the hemN gene of L. lactis NZ9000; Ermr This work
 pTHCas9recA1 pTHCas9 with a 20 bp spacer targeting the recA gene of L. lactis NZ9000; Ermr This work
 pTHCas9noxD1 pTHCas9 with a 20 bp spacer targeting the noxD gene of L. lactis NZ9000; Ermr This work
 pTHCas9galk2 pTHCas9 with a 25 bp spacer targeting the galK gene of L. lactis NZ9000; Ermr This work
 pTHCas9hemN2 pTHCas9 with a 25 bp spacer targeting the hemN gene of L. lactis NZ9000; Ermr This work
 pTHCas9recA2 pTHCas9 with a 25 bp spacer targeting the recA gene of L. lactis NZ9000; Ermr This work
 pTHCas9noxD2 pTHCas9 with a 25 bp spacer targeting the noxD gene of L. lactis NZ9000; Ermr This work
 pTHCas9galk3 pTHCas9 with a 30 bp spacer targeting the galK gene of L. lactis NZ9000; Ermr This work
 pTHCas9hemN3 pTHCas9 with a 30 bp spacer targeting the hemN gene of L. lactis NZ9000; Ermr This work
 pTHCas9recA3 pTHCas9 with a 30 bp spacer targeting the recA gene of L. lactis NZ9000; Ermr This work
 pTHCas9noxD3 pTHCas9 with a 30 bp spacer targeting the noxD gene of L. lactis NZ9000; Ermr This work
 pTHCas9upp pTHCas9 with a 25 bp spacer targeting the upp gene of L. lactis NZ9000; Ermr This work
 pTHCas9galk pTHCas9 with a 25 bp spacer targeting the galk gene of L. lactis NZ9000; Ermr This work
 pL-beta λ Red beta cloning in pLeiss:Sec:Nuc; Cmr This work
 pL-RecT RecT from E. faecalis ATCC14506 cloning in pLeiss:Sec:Nuc; Cmr This work
 pL-LC50 LCABL_13050 from Lb. casei BL23 cloning in pLeiss:Sec:Nuc; Cmr This work
 pL-Lp41 Lp_0641 from Lb. plantarum WCFS1 cloning in pLeiss:Sec:Nuc; Cmr This work
 pL-JB02 phiJB_00020 from Lb. delbrueckii SDMCC050201 cloning in pLeiss:Sec:Nuc; Cmr This work
 pL-pi12 pi12 from L. lactis IL1403 cloning in pLeiss:Sec:Nuc; Cmr This work