Fig. 5

Short DNA fragment deletion from the L. lactis genome. a Schematic diagram of deletion of partial noxD gene in L. lactis NZ9000. b, c PCR amplification of the noxD locus from randomly selected colonies surviving under erythromycin selection. The wild type corresponds to an amplicon 240 bp in size, whereas the 50 bp and 100 bp in-frame deletion result in products at 190 bp (b) and 140 bp (c) in size. DL 5,000 ladder (Takara, Japan) is shown