Fig. 1
From: Exploring natural biodiversity to expand access to microbial terpene synthesis
Phylogenetic tree of 82 cloned IPK candidates from biodiversity compared with IPKs from literature. Bootstrap values expressed as a percentage of 1000 replications are shown next to each node if value > 75%. a UniProt identifiers of each candidates. The 4 IPKs from literature used as the reference set in the sequence driven approach are noted in blue. b Best hit from IPK reference set and its percentage of identity and homology with the candidate. c Phylogenetic lineage of IPKs: Archaea are in blue (light blue: Euryarchaeota; dark blue: Crenarchaeota) and bacteria are in green (light green: Proteobacteria; dark green: Terrabacteria group). d Selected microorganisms. e Protein expression status according SDS gel analysis of cell free extract by eye. “I” full red dot means that the protein was visibly induced by IPTG treatment and “L” means that the protein is visibly present in the cell free extract. f Quantification by LC/MS analysis of IPP/DMAPP production (µM/L) in 96-microwell screening. g Protein name according to UniProt annotation