Table 3 Kinetic and stoichiometric parameters of E. coli W3110 and derived glucose transport mutants expressing GFP in stirred tank bioreactors
Strain | µ (h−1) | qs (g/g h) | qac (g/g h) | Max acetate (g/L) | Y X/S (g/g) | Max GFP (mg/L) | qGFP (mg/g h) | Y GFP/X (mg/g) | Max Biomass (g/L) | Culture time (h) |
|---|---|---|---|---|---|---|---|---|---|---|
W3110/pV21 | 0.52 ± 0.01 | 1.65 ± 0.06 | 0.112 ± 0.064 | 0.49 ± 0.10 | 0.24 ± 0.01 | 50.5 ± 4.6 | 2.5 ± 0.1 | 10.5 ± 0.9 | 5.1 ± 0.4 | 12 |
WG/pV21 | 0.35 ± 0.00 | 1.11 ± 0.16 | 0.012 ± 0.003 | 0.26 ± 0.03 | 0.25 ± 0.01 | 342.0 ± 35.0 | 9.3 ± 1.7 | 71.8 ± 4.5 | 4.7 ± 0.4 | 22 |
WGM/pV21 | 0.27 ± 0.00 | 0.83 ± 0.03 | 0.005 ± 0.002 | 0.16 ± 0.02 | 0.26 ± 0.01 | 438.5 ± 6.6 | 12.8 ± 0.3 | 91.3 ± 0.7 | 4.8 ± 0.1 | 34 |
WGX/pV21 | 0.24 ± 0.01 | 0.75 ± 0.07 | 0.003 ± 0.000 | 0.15 ± 0.03 | 0.24 ± 0.01 | 231.9 ± 34.7 | 6.7 ± 0.9 | 49.2 ± 4.9 | 4.7 ± 0.3 | 26 |
WGMC/pV21 | 0.23 ± 0.02 | 0.66 ± 0.04 | 0.003 ± 0.000 | 0.17 ± 0.04 | 0.27 ± 0 | 252.8 ± 11.0 | 7.1 ± 0.8 | 52.2 ± 2.1 | 4.8 ± 0.1 | 35 |
WHI/pV21 | 0.15 ± 0.01 | 0.44 ± 0.02 | 0.008 ± 0.002 | 1.05 ± 0.01 | 0.22 ± 0.02 | 187.6 ± 44.2 | 3.1 ± 0.7 | 42.8 ± 9.6 | 4.3 ± 0.3 | 40 |
WHIC/pV21 | 0.16 ± 0.01 | 0.45 ± 0.00 | 0.011 ± 0.011 | 0.52 ± 0.06 | 0.25 ± 0.01 | 158.8 ± 21.3 | 2.5 ± 0.3 | 37.5 ± 5.6 | 4.3 ± 0.5 | 40 |