Table 2 Kinetic and stoichiometric parameters of E. coli W3110 and derived glucose transport mutants expressing GFP in shake-flask cultures
Strain | µ (h−1) | qs (g/g h) | qac (g/g h) | Max acetate (g/L) | Y X/S (g/g) | Max GFP (mg/L) | qGFP (mg/g h) | Y GFP/X (mg/g) | Max biomass (g/L) | Culture time (h) |
|---|---|---|---|---|---|---|---|---|---|---|
W3110/pV21 | 0.54 ± 0.01 | 1.75 ± 0.05 | 0.47 ± 0.03 | 0.54 ± 0.00 | 0.30 ± 0.02 | 11.9 ± 0.4 | 8.0 ± 0.5 | 7.7 ± 0.2 | 1.58 ± 0.04 | 10 |
WG/pV21 | 0.34 ± 0.00 | 0.90 ± 0.06 | 0.01 ± 0.00 | 0.06 ± 0.01 | 0.27 ± 0.01 | 305.4 ± 8.6 | 54.2 ± 1.3 | 211.5 ± 8.9 | 1.45 ± 0.03 | 14 |
WGX/pV21 | 0.30 ± 0.00 | 0.66 ± 0.02 | 0.04 ± 0.00 | 0.35 ± 0.03 | 0.27 ± 0.00 | 138.3 ± 25.9 | 19.5 ± 0.5 | 71.2 ± 18.9 | 1.94 ± 0.03 | 21 |
WGM/pV21 | 0.25 ± 0.00 | 0.59 ± 0.01 | 0.08 ± 0.00 | 1.06 ± 0.1 | 0.3 ± 0.00 | 153.2 ± 29.4 | 8.9 ± 0.1 | 83 ± 15.8 | 1.97 ± 0.03 | 21 |
WGMC/pV21 | 0.24 ± 0.00 | 0.53 ± 0.05 | 0.06 ± 0.01 | 1.19 ± 0.16 | 0.31 ± 0.01 | 55.1 ± 10.6 | 8.1 ± 0.1 | 27.5 ± 7.3 | 2.11 ± 0.07 | 21 |
WHI/pV21 | 0.17 ± 0.01 | 0.45 ± 0.03 | 0.004 ± 0.001 | 0.03 ± 0.01 | 0.29 ± 0.02 | 108.3 ± 22.9 | 13.6 ± 1.4 | 65.4 ± 15.8 | 1.93 ± 0.04 | 28 |
WHIC/pV21 | 0.16 ± 0.01 | 0.45 ± 0.02 | NDa | 0.00 ± 0.00 | 0.32 ± 0.02 | 150.7 ± 20.3 | 12.8 ± 2.2 | 97.6 ± 19.0 | 1.68 ± 0.15 | 32 |