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Table 1 Physiological parameters calculated for control and strain xfpk(BB) in batch and chemostat mode

From: Heterologous phosphoketolase expression redirects flux towards acetate, perturbs sugar phosphate pools and increases respiratory demand in Saccharomyces cerevisiae

Strain

Batch

Chemostat

Control

xfpk(BB)

Control

xfpk(BB)

µ(max) (h−1)

0.353 ± 0.008

0.266 ± 0.006

0.104 ± 0.002

0.103 ± 0.003

Y(x/s) (gCDW/gGlucose)

0.135 ± 0.00

0.123 ± 0.00

0.479 ± 0.01

0.409 ± 0.01

q(Glucose) (mmol gCDW−1 h−1)

− 14.42 ± 0.36

− 12.19 ± 0.39

− 1.18 ± 0.06

− 1.36 ± 0.03

q(EtOH) (mmol gCDW−1 h−1)

21.46 ± 0.75

17.26 ± 0.52

–

–

q(Acetate) (mmol gCDW−1 h−1)

0.61 ± 0.04

1.38 ± 0.03

–

–

q(Glycerol) (mmol gCDW−1 h−1)

1.13 ± 0.03

0.62 ± 0.04

–

–

q(Pyruvate) (mmol gCDW−1 h−1)

0.13 ± 0.01

0.15 ± 0.00

–

–

q(Succinate) (mmol gCDW−1 h−1)

0.02 ± 0.0

0.01 ± 0.0

–

–

q(CO2) (mmol gCDW−1 h−1)

30.01 ± 0.32

28.16 ± 0.97

2.91 ± 0.19

3.34 ± 0.09

q(Biomass) (mmol gCDW−1 h−1)

14.37 ± 0.31

10.81 ± 0.25

4.13 ± 0.31

4.07 ± 0.25

q(O2) (mmol gCDW−1 h−1)

− 5.49 ± 0.53

− 7.63 ± 0.76

− 2.61 ± 0.20

− 3.35 ± 0.12

Carbon balance

107% ± 2%

107% ± 2%

99% ± 3%

92% ± 1%

  1. Strains were grown in biological quadruplicates in minimal media with 2% glucose during batch phase and 0.75% glucose in the feed in chemostat conditions. Values shown correspond to averages ± standard deviation