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Table 1 Physiological parameters calculated for control and strain xfpk(BB) in batch and chemostat mode

From: Heterologous phosphoketolase expression redirects flux towards acetate, perturbs sugar phosphate pools and increases respiratory demand in Saccharomyces cerevisiae

Strain Batch Chemostat
Control xfpk(BB) Control xfpk(BB)
µ(max) (h−1) 0.353 ± 0.008 0.266 ± 0.006 0.104 ± 0.002 0.103 ± 0.003
Y(x/s) (gCDW/gGlucose) 0.135 ± 0.00 0.123 ± 0.00 0.479 ± 0.01 0.409 ± 0.01
q(Glucose) (mmol gCDW−1 h−1) − 14.42 ± 0.36 − 12.19 ± 0.39 − 1.18 ± 0.06 − 1.36 ± 0.03
q(EtOH) (mmol gCDW−1 h−1) 21.46 ± 0.75 17.26 ± 0.52 – –
q(Acetate) (mmol gCDW−1 h−1) 0.61 ± 0.04 1.38 ± 0.03 – –
q(Glycerol) (mmol gCDW−1 h−1) 1.13 ± 0.03 0.62 ± 0.04 – –
q(Pyruvate) (mmol gCDW−1 h−1) 0.13 ± 0.01 0.15 ± 0.00 – –
q(Succinate) (mmol gCDW−1 h−1) 0.02 ± 0.0 0.01 ± 0.0 – –
q(CO2) (mmol gCDW−1 h−1) 30.01 ± 0.32 28.16 ± 0.97 2.91 ± 0.19 3.34 ± 0.09
q(Biomass) (mmol gCDW−1 h−1) 14.37 ± 0.31 10.81 ± 0.25 4.13 ± 0.31 4.07 ± 0.25
q(O2) (mmol gCDW−1 h−1) − 5.49 ± 0.53 − 7.63 ± 0.76 − 2.61 ± 0.20 − 3.35 ± 0.12
Carbon balance 107% ± 2% 107% ± 2% 99% ± 3% 92% ± 1%
  1. Strains were grown in biological quadruplicates in minimal media with 2% glucose during batch phase and 0.75% glucose in the feed in chemostat conditions. Values shown correspond to averages ± standard deviation
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Microbial Cell Factories

ISSN: 1475-2859

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