Comparative proteome analysis in an Escherichia coli CyDisCo strain identifies stress responses related to protein production, oxidative stress and accumulation of misfolded protein

Table 2 Comparison of the growth rate and doubling time of the exponential phase and maximum OD reached by the cultures after 7 h

Strains	Specific growth rate* (h⁻¹)	Doubling time* (h)	Maximum OD (at 600 nm)
Induced no POI	0.75 ± 0.018	0.92 ± 0.022	2.08
Induced ScFv	0.74 ± 0.005	0.94 ± 0.006	1.71
Induced misfolded	0.69 ± 0.013	1.01 ± 0.019	1.64
Uninduced no POI	0.66 ± 0.022	1.05 ± 0.035	2.52
Uninduced ScFv	0.69 ± 0.019	1.01 ± 0.028	2.23
Uninduced misfolded	0.72 ± 0.016	0.96 ± 0.022	2.70
Empty	0.76 ± 0.018	0.91 ± 0.021	3.73

Cultivations were performed at 30 °C and 200 rpm for all conditions. The cells were grown aerobically in LB medium for 7 h and induced with IPTG after 2.5 h. The data for the calculation of the growth rate and generation time during the exponential phase was taken from time points at 1.5 h and 4.5 h
* Average from three replicates, ± standard deviation

ISSN: 1475-2859