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Fig. 6 | Microbial Cell Factories

Fig. 6

From: Comparative proteome analysis in an Escherichia coli CyDisCo strain identifies stress responses related to protein production, oxidative stress and accumulation of misfolded protein

Fig. 6

The E. coli CyDisCo strain expressing misfolded scFv shows Ag43-mediated cell aggregation. Phase-contrast microscopy images of CyDisCo: control (a1), scFv (a2) and misfolded scFv (a3) expressing strains. Samples were harvested 3 h post-induction with IPTG. b Sedimentation profile of E. coli CyDisCo strains. Cultures were grown for 3 h post-induction, transferred into tubes and incubated statically for 24 h. c Percentage of aggregation as quantified from the change in OD600 over 24 h from the experiment performed as in b

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