Production of long-chain free fatty acids from metabolically engineered Rhodobacter sphaeroides heterologously producing periplasmic phospholipase A2 in dodecane-overlaid two-phase culture

Table 3 Carbon-to-lipid conversion yield, FFA titer, and FFA productivity of Rs-HCA2 and Rs-HXYCA2

	Rs-HCA2		Rs-HXYCA2
	Dodecane
	–	+	–	+
Carbon-to-lipid conversion yield (g g⁻¹)^a	0.14 ± 0.02^d	0.17 ± 0.01	0.18 ± 0.01	0.20 ± 0.01
Titer (g L⁻¹)^b	2.6 ± 0.2	6.8 ± 0.5	3.4 ± 0.5	8.5 ± 0.7
Productivity (g L⁻¹ day⁻¹)^c	0.7 ± 0.2	2.0 ± 0.1	1.0 ± 0.2	2.8 ± 0.2

^aRatio of total lipids to the carbon sources (glucose, glycerol, and succinate) consumed by the recombinant strains (Additional file 1: Fig. S16), calculated as described previously [62]
^bMaximum FFA level per liter of culture medium
^cProductivity at the exponential growth phase (OD₆₆₀ of 6.5 and 17.5 for Rs-HCA2 in the absence and the presence of dodecane, respectively; OD₆₆₀ of 6.9 and 14.0 for Rs-HXYCA2 in the absence and the presence of dodecane, respectively)
^dMean ± SD

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