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Table 3 Carbon-to-lipid conversion yield, FFA titer, and FFA productivity of Rs-HCA2 and Rs-HXYCA2

From: Production of long-chain free fatty acids from metabolically engineered Rhodobacter sphaeroides heterologously producing periplasmic phospholipase A2 in dodecane-overlaid two-phase culture

  Rs-HCA2 Rs-HXYCA2
Dodecane
+ +
Carbon-to-lipid conversion yield (g g−1)a 0.14 ± 0.02d 0.17 ± 0.01 0.18 ± 0.01 0.20 ± 0.01
Titer (g L−1)b 2.6 ± 0.2 6.8 ± 0.5 3.4 ± 0.5 8.5 ± 0.7
Productivity (g L−1 day−1)c 0.7 ± 0.2 2.0 ± 0.1 1.0 ± 0.2 2.8 ± 0.2
  1. aRatio of total lipids to the carbon sources (glucose, glycerol, and succinate) consumed by the recombinant strains (Additional file 1: Fig. S16), calculated as described previously [62]
  2. bMaximum FFA level per liter of culture medium
  3. cProductivity at the exponential growth phase (OD660 of 6.5 and 17.5 for Rs-HCA2 in the absence and the presence of dodecane, respectively; OD660 of 6.9 and 14.0 for Rs-HXYCA2 in the absence and the presence of dodecane, respectively)
  4. dMean ± SD