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Table 2 Expression of fabH, plsX, plsY, plsC, and pla2 in Rs-HCA2 and Rs-HXYCA2

From: Production of long-chain free fatty acids from metabolically engineered Rhodobacter sphaeroides heterologously producing periplasmic phospholipase A2 in dodecane-overlaid two-phase culture

Genesb Strainsa
WT Rs-HCA2 Rs-HXYCA2
fabH 1.0 ± 0.7 64.7 ± 0.2d (0.007)e 52.4 ± 0.1d (0.032)e
plsX 1.0 ± 0.1 0.6 ± 0.1d (0.04)e 15.1 ± 0.7d (0.001)e
plsY 1.0 ± 0.1 0.2 ± 0.1d (0.012)e 89.0 ± 0.7d (0.001)e
plsC 1.0 ± 0.1 6.4 ± 0.4d (0.004)e 6.2 ± 0.2d (0.004)e
pla2 NAc 1.0 ± 0.4 1.1 ± 0.1f (0.3)e
  1. aCells were grown photoheterotrophically in SisH medium and harvested at the exponential phase (OD660 of 9.5, 5.3 and 5.4 for WT, Rs-HCA2 and Rs-HXYCA2, respectively)
  2. bExpression levels were examined by RT-qPCR
  3. cNot applicable
  4. dMean ratio (± SD) to the expression of the corresponding gene in WT cells, which was set to 1, from three independent experiments with 16S rRNA as a reference gene
  5. eSignificance level regarded as significant when the value is less than 0.05
  6. fMean ratio (± SD) to the expression of pla2 in Rs-HCA2, which was set to 1, from three independent experiments with 16S rRNA as a reference gene