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Table 1 Strains and plasmids used in this study

From: Production of long-chain free fatty acids from metabolically engineered Rhodobacter sphaeroides heterologously producing periplasmic phospholipase A2 in dodecane-overlaid two-phase culture

Strains/plasmids Relevant characteristics References/description
Strains
 E. coli
  DH5αphe phe::Tn10dCm of DH5α [32]
  HB101 F mcrB mrr hsdS20(r B m B ) recA13 leuB6 ara-14 proA2 lacY1 galK2 xyl-5 mtl-1 rpsL20(SmR) glnV44 λ [33]
  S17-1 C600::RP4 2-(Tc::Mu)(Km::Tn7) thi pro hsdR hsdM+ recA [34]
 R. sphaeroides
  KD131 Type strain [25]
  Rs-nA2 R. sphaeroides containing pIND-PLA2 This study
  Rs-A2 R. sphaeroides containing pINDA′-PLA2 This study
  Rs-nA2-phoA R. sphaeroides containing pIND-PLA2-phoA This study
  Rs-A2-phoA R. sphaeroides containing pINDA′-PLA2-phoA This study
  Rs-C R. sphaeroides containing pLVplsC This study
  Rs-DC R. sphaeroides containing pRKfabD and pLVplsC This study
  Rs-AccC R. sphaeroides containing pRKaccABCD and pLVplsC This study
  Rs-HC R. sphaeroides containing pRKfabH and pLVplsC This study
  Rs-AccCA2 R. sphaeroides containing pRKaccABCD, pLVplsC and pINDA′-PLA2 This study
  Rs-CA2 R. sphaeroides containing pLVplsC and pINDA′-PLA2 This study
  Rs-DCA2 R. sphaeroides containing pRKfabD, pLVplsC and pINDA′-PLA2 This study
  Rs-HCA2 R. sphaeroides containing pRKfabH, pLVplsC and pINDA′-PLA2 This study
  Rs-HXYCA2 R. sphaeroides containing pRKfabH, pBBRplsX, pLVplsCY and pINDA′-PLA2 This study
  Rs-tol A tolC mutant; Tpr This study
  Rs-A2tol A tolC mutant containing pINDA′-PLA2; Tpr This study
Plasmids
 pBBR1MCS-2 ori pBBR1 lacZa; Kmr [35]
 pBBRplsX pBBR + 1.3-kb HindIII–XbaI fragment containing PlsX; Kmr This study
 pBS(−) ori f1 lacZ′; Apr Stratagene
 pBSaccA pBS + 1.1-kb XbaI–PstI fragment containing accA; Apr This study
 pBSaccD pBS + 1.3-kb XbaI–PstI fragment containing accD; Apr This study
 pBSfabD pBS + 1.2-kb XbaI–PstI fragment containing fabD; Apr This study
 pMD20 ori f1 lacZ′; Apr Takara
 pMDaccBC pMD + 2.9-kb HindIII–XbaI fragment containing accBC; Apr This study
 pIND4 ori ColE1 IPTG-inducible promoter; Kmr [29]
 pIND4S ori ColE1 IPTG-inducible promoter; Sm/Spr This study
 pIND-PLA2 pIND4S + 387-bp NcoI–HindIII fragment containing PLA2; Sm/Spr This study
 pINDA′-PLA2 pIND4S + 456-bp NcoI–HindIII fragment containing cycA′-PLA2; Sm/Spr This study
 pINDA′-PLA1 pIND4S + 1.9-kb NcoI–HindIII fragment containing cycA′-PLA1; Sm/Spr This study
 pIND-PLA2-phoA pIND4S + 1.9-kb NcoI–HindIII fragment containing PLA2-phoA; Sm/Spr This study
 pINDA′-PLA2-phoA pIND4S + 2.0-kb NcoI–HindIII fragment containing cycA′-PLA2-phoA; Sm/Spr This study
 pLV106 IncQ pUI511 containing the polylinker of EcoRI, SmaI, KpnI, and XbaI restriction sites inserted at PstI site [36]
 pLV-Tp pLV106 + 1.6-kb HindIII fragment containing Tpr gene This study
 pLVplsC pLV-Tp + 1.0-kb XbaI–KpnI fragment containing plsC; Tpr This study
 pLVplsCY pLV-Tp + 1.0-kb XbaI–KpnI fragment containing plsC and 0.8-kb KpnI-EcoRI fragment containing plsY; Tpr This study
 pRK2013 ori ColE1; Kmr [37]
 pRK415 ori IncP-1 Mob RP4 lacZa; Tcr [38]
 pRKfabH pRK415 + 1.8-kb KpnI–XbaI fragment containing fabH; Tcr This study
 pRKfabD pRK415 + 1.2-kb XbaI–KpnI fragment containing fabD; Tcr This study
 pRKaccABCD pRK415 + 5.3-kb XbaI–KpnI fragment containing accABCD; Tcr This study
 pSUP202 ori p15A Mob RP4; Tcr Cmr Apr [39]
 pSUPTolC pSUP202 + 2.6-kb DNA of tolC::Tp; tolC interrupted by 1.6-kb Tpr DNA; Tpr Tcr This study