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Fig. 8 | Microbial Cell Factories

Fig. 8

From: Production of long-chain free fatty acids from metabolically engineered Rhodobacter sphaeroides heterologously producing periplasmic phospholipase A2 in dodecane-overlaid two-phase culture

Fig. 8

FFA productivities of Rs-HCA2 and Rs-HXYCA2 grown at high cell densities in a two-phase system with dodecane. a1 Rs-HCA2 and a2 Rs-HXYCA2 were grown photoheterotrophically in SisH-D medium with or without 30% dodecane (v/v). The culture broth and dodecane layer were harvested during growth, and the FFAs in the culture supernatant (open bar) and the dodecane layer (closed bar) were measured. The FFA productivity is illustrated with error bars from three independent experiments. The numbers 1–5 correspond to the time points of harvesting the culture broth and dodecane layer from b1 and b2. b1 Rs-HCA2 and b2 Rs-HXYCA2 were cultured in SisH-D medium with or without 30% dodecane (v/v) under photoheterotrophic conditions; data shown are for one of three representative experiments

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Microbial Cell Factories

ISSN: 1475-2859

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