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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Production of long-chain free fatty acids from metabolically engineered Rhodobacter sphaeroides heterologously producing periplasmic phospholipase A2 in dodecane-overlaid two-phase culture

Fig. 3

PLA2 activities of the periplasmic and cytoplasmic fractions of Rs-A2. Rs-A2 was grown photoheterotrophically in Sis minimal medium. Periplasmic and cytoplasmic fractions were prepared from cells harvested at the exponential growth phase (OD660 of 2.3). The PLA activity in each fraction was determined with membrane lipids from the photoheterotrophically grown WT cells and expressed as the percentage of the total activity, which amounted to approximately 111.5 U mg−1 protein. One enzyme unit was defined as 1 µmol of FFAs released per min from PLs under the assay conditions examined. PLA2 activities are shown with error bars from three independent experiments

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