Fig. 2

Heterologous production of PLA2 in the periplasm of Rs-A2 and its FFA productivity. a PhoA activity of Rs-A2-phoA was examined with BCIP/NBT solution, which was dropped onto colonies grown aerobically on Sis minimal medium agar plates and incubated at 30 °C for 5 min (right). Rs-nA2-phoA colonies were used as a control (left). b R. sphaeroides heterologously producing PLA2 in either the cytoplasm (Rs-nA2) or periplasm (Rs-A2) was grown photoheterotrophically and harvested at the exponential growth phase (OD₆₆₀ of 2.3). The FFA level in the culture supernatant was measured, and the FFA productivity is shown with error bars from three independent experiments