Table 1 Plasmids and strains used in this study

pZE-EP

entC, pchB, and luc genes under control of P_lac; Amp^R

[14]

pCS-APTA

aroL ppsA, tktA, aroG^fbr under control of P_lac; Kan^R

[14]

pPCC1115-5

CRIM plasmid with P_BAD-lacZ;;Apr^R

[21, 29]

pPCC1244

araC-SA gene under the control of P_tac; Apr^R

In this study

pPCC1250

entC, pchB genes under control of P_tac; Amp^R

In this study

pPCC1251

araC-SA, entC, pchB genes under control of P_tac; Apr^R

In this study

pPCC1252

aroL ppsA, tktA, aroG^fbr genes under control of P_tac; Apr^R

In this study

pPCC1253

araC-SA, entC, pchB genes under control of P_tac; aroL ppsA, tktA, aroG^fbr genes under control of P_tac; Apr^R

In this study

pPCC1253-aroL

araC-SA, entC, pchB genes under control of P_tac; ppsA, tktA, aroG^fbr genes under control of P_tac; Apr^R

In this study

pPCC507

From vector pJA1. Transposable element containing a chloramphenicol resistance gene flanked by IS10 inverted repeat sequences; R6 K replication origin; tnp gene encoding a mutant Tn10 transposase with 100-fold lower frequency of insertion at hot spots; Amp^R

[21, 31]

pFG1

araC gene under the control of P_tac, tac; Apr^R

[18]

pFG29-SA

araC-SA gene under the control of P_tac; gfpuv under control of P_BAD promoter, Apr^R

[20]

Strains

HF19

BW27786 (∆araFGH, ∆araBAD, ∆lacZ), with araC deleted

[16]

QH4

ATCC31884 with pheA and tyrA disrupted

[14]

SQ18

QH4 with P_BAD-lacZ reporter integrated into chromosome at HK022 site

In this study

SQ22

SQ18 with LacZ in the lac operon disrupted

In this study

QH4Δrnd

QH4 with rnd gene disrupted

In this study

SQ22Δrnd

SQ22 with rnd gene disrupted

In this study