Fig. 2
From: Biosensor-guided improvements in salicylate production by recombinant Escherichia coli
Development of a salicylate-responsive sensor-reporter system. a Heterologous pathway for producing salicylate from the native E. coli central metabolite chorismate. b Fold activation of GFP expression under control of AraC-SA/PBAD, in the presence of 5 mM salicylate (SA) and similar compounds (l-ara: l-arabinose (inducer for wild-type AraC); BA: benzoic acid; 2-MOBA: 2-methoxybenzoic acid; 3-HBA: 3-hydroxybenzoic acid; 4-HBA: 4-hydroxybenzoic acid; and 2MBA: 2-methylbenzoic acid. Reported data represents mean ± std. dev., from four biological replicates. c Response of AraC sensor in E. coli strain QH4 carrying plasmid pGF29-SA (neither entC nor pchB is expressed), plasmid pFG29-SA-entC (entC but not pchB are expressed), and plasmid pFG29-SA-EP (enabling salicylate production). d Colorimetric colony assay using the designed salicylate sensor system (SQ22 expressing AraC-SA growing on LB-agar plates containing X-GAL and different concentrations of salicylate)