Engineering an electroactive Escherichia coli for the microbial electrosynthesis of succinate from glucose and CO2

Table 3 MES fermentation profile of E. coli T110, T110 (pMtrABC, pFccA-CymA) and T110 (pMtrABC, pFccA-CymA, pBTCA)

Characteristics	Consumed Glucose (mM)	Product concentration (mM) and yields without electricity		Product concentration (mM) and yields with MES
Characteristics	Consumed Glucose (mM)	Succinate	Acetate	Succinate	Acetate
T110	27.28 ± 0.52	16.64 ± 0.36^a	13.47 ± 0.12	18.55 ± 0.38	13.91 ± 0.07
Yield (mol/mol glucose)	27.28 ± 0.52	0.61 ± 0.05	0.48 ± 0.03	0.68 ± 0.05	0.51 ± 0.03
T110 (pMtrABC, pFccA-CymA)	27.78 ± 0.52	15.56 ± 0.40	14.68 ± 0.32	26.39 ± 1.20	11.11 ± 0.75
Yield (mol/mol glucose)	27.78 ± 0.52	0.56 ± 0.03	0.53 ± 0.02	0.95 ± 0.04	0.40 ± 0.02
T110 (pMtrABC, pFccA-CymA, pBTCA)	27.78 ± 0.52	18.06 ± 0.56	14.10 ± 0.50	30.56 ± 1.26	9.16 ± 0.20
Yield (mol/mol glucose)	27.78 ± 0.52	0.65 ± 0.08	0.51 ± 0.04	1.10 ± 0.03	0.33 ± 0.01

100 mM KHCO₃ was added into each MES fermentation using 27.78 mM glucose as the substrate, room temperature, 200–250 rpm, 7 days, constant sparging with pure N₂ to ensure strict anaerobic conditions in the MES system
^aThe data is the mean of three replicates, present with standard deviation

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