Engineering an electroactive Escherichia coli for the microbial electrosynthesis of succinate from glucose and CO2

Table 2 MES fermentation profile of E. coli ATCC8739(pMtrABC, pFccA-CymA)

Characteristics	Concentration (mM) of the following fermentation products
Characteristics	Succinate	Lactate	Formate	Acetate	Ethanol
8739 (pMtrABC, pFccA-CymA) without electricity	1.31 ± 0.02^a	5.43 ± 0.03	1.06 ± 0.02	32.21 ± 0.50	34.68 ± 0.70
Yield (mol/mol Glucose)	0.02 ± 0.00	0.10 ± 0.00	0.02 ± 0.00	0.58 ± 0.04	0.63 ± 0.05
8739 (pMtrABC, pFccA-CymA) with electricity	2.17 ± 0.01	11.96 ± 0.20	1.60 ± 0.04	24.53 ± 1.00	53.31 ± 1.60
Yield (mol/mol Glucose)	0.04 ± 0.01	0.22 ± 0.01	0.03 ± 0.00	0.44 ± 0.08	0.96 ± 0.08

55.56 mM glucose was consumed in each MES fermentation, room temperature, 200–250 rpm, 7 days, constant sparging with pure N₂ to ensure strict anaerobic conditions in the MES system
^aThe data is the mean of three replicates, present with standard deviation

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