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Table 1 E. coli strains and plasmids used in this research

From: Engineering an electroactive Escherichia coli for the microbial electrosynthesis of succinate from glucose and CO2

Strains and plasmids

Characteristics

Sources

ATCC8739

Wild type

Lab collection

MG1655

Wild type

Lab collection

T110

ATCC8739, a∆ldhA, ∆pflB, ∆ptsI, Ppck*-galP, Ppck*-pck

Tan et al. [46]

pACYC184

Cm, p15A origin of replication, lacI

Lab collection

pTrac99A-apr

Apr, pMB1 origin of replication, lacI

Lab collection

pTrac99A-spe

Str, pMB1 origin of replication

Lab collection

pTrac99A-M

Amp, pMB1 origin of replication, lacI

Lab collection

pBTCA

carbonic anhydride (CA) encoding gene (ccaA) and bicarbonate transporter (BT) encoding gene (bicA) in pTrac99A-M

Lab collection

pBAD-rfp

Kan, pBBR1 origin of replication, BBa J23100

Lab collection

pMtrABC

pACYC184 derived plasmid expressing the outer membrane protein complex encoding genes mtrA-mtrB-mtrC from S. oneidensis

This study

pFccA-CymA

pTrac99A-apr derived plasmid expressing cymA and fccA from S. oneidensis

This study

pFccA

pTrac99A-spe and pTrac99A-M derived plasmid expressing fccA from S. oneidensis

This study

pMtrA-RFP

pACYC184 derived plasmid expressing the fusion protein of MtrA-RFP

This study

pCymA-RFP

pTac99A-apr derived plasmid expressing fusion protein of CymA-RFP

This study

pFccA-RFP

pTrac99A-apr derived plasmid expressing fusion protein of FccA-RFP

This study

  1. aâ–³ represents the inactivated genes by gene deletion
  2. * Represent the activate genes by gene regulation