Table 1 Strains used in this study
Strains | Genotype and/or relevant characteristic (s) | Source |
|---|---|---|
E. coli DH5α | F−ΔlacU169(Ø80d lacZΔM15) supE44 hsdR17 recA1 gyrA96 endA1 thi-1 relA1 | Invitrogen |
B. subtilis 1A751 | eglS∆102 bglT/bglS∆EV aprE nprE his | BGSC |
Kno6 | eglS∆102 bglT/bglS∆EV aprE nprE his ∆bpr∆epr∆HtrA∆mpr∆ nprB∆vpr | This work |
1A751C | 1A751 containing pMATE; Kmr | This work |
1A751F1 | 1A751 containing pMATEF; Kmr | This work |
1A751F2 | 1A751 containing pMA5F; Kmr | This work |
CIS1 | 1A751 containing pMATEFc1; Kmr | This work |
CIS2 | 1A751 containing pMATEFc2; Kmr | This work |
CIS3 | 1A751 containing pMATEFc3; Kmr | This work |
CIS4 | 1A751 containing pMATEFc4; Kmr | This work |
CIS5 | 1A751 containing pMATEFc5; Kmr | This work |
CIS6 | 1A751 containing pMATEFc6; Kmr | This work |
CIS7 | 1A751 containing pMATEFc7; Kmr | This work |
CHAP1 | CIS5 containing pDF1; Cmr, Kmr | This work |
CHAP 2 | CIS5 containing pDF2; Cmr, Kmr | This work |
CHAP 3 | CIS5 containing pDF3; Cmr, Kmr | This work |
CHAP 4 | CIS5 containing pDF4; Cmr, Kmr | This work |
CHAP 5 | CIS5 containing pDF5; Cmr, Kmr | This work |
CHAP 6 | CIS5 containing pDF6; Cmr, Kmr | This work |
CHAP 7 | CIS5 containing pDF7; Cmr, Kmr | This work |
CHAP 8 | CIS5 containing pDF8; Cmr, Kmr | This work |
CHAP 9 | CIS5 containing pDF9; Cmr, Kmr | This work |
CHAP 10 | CIS5 containing pDF10; Cmr, Kmr | This work |
CHAP 11 | CIS5 containing pDF11; Cmr, Kmr | This work |
CHAP3R1 | CHAP3 containing pMATEFc5R1; Cmr, Kmr | This work |
CHAP3R2 | CHAP3 containing pMATEFc5R2; Cmr, Kmr | This work |
CHAP3R3 | CHAP3 containing pMATEFc5R3; Cmr, Kmr | This work |
Kno6cs | Kno6 containing pMATEFc5; Kmr | This work |
Kno6cf | Kno6 containing pDF3, pMATEFc5; Cmr, Kmr | This work |
Kno6k | Kno6 containing pDF3; Cmr | This work |
Kno6csp 1 | Kno6k containing pMATEF1; Cmr, Kmr | This work |
Kno6csp 2 | Kno6k containing pMATEF2; Cmr, Kmr | This work |
Kno6csp 3 | Kno6k containing pMATEF3; Cmr, Kmr | This work |
Kno6csp 4 | Kno6k containing pMATEF4; Cmr, Kmr | This work |
Kno6csp 5 | Kno6k containing pMATEF5; Cmr, Kmr | This work |
Kno6csp 6 | Kno6k containing pMATEF6; Cmr, Kmr | This work |
Kno6csp 7 | Kno6k containing pMATEF7; Cmr, Kmr | This work |
Kno6csp 8 | Kno6k containing pMATEF8; Cmr, Kmr | This work |
Kno6csp 9 | Kno6k containing pMATEF9; Cmr, Kmr | This work |
Kno6csp 10 | Kno6k containing pMATEF10; Cmr, Kmr | This work |
Kno6csp 11 | Kno6k containing pMATEF11; Cmr, Kmr | This work |