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Table 1 Strains used in this study

From: High-efficiency expression and secretion of human FGF21 in Bacillus subtilis by intercalation of a mini-cistron cassette and combinatorial optimization of cell regulatory components

Strains Genotype and/or relevant characteristic (s) Source
E. coli DH5α F−ΔlacU169(Ø80d lacZΔM15) supE44 hsdR17 recA1 gyrA96 endA1 thi-1 relA1 Invitrogen
B. subtilis 1A751 eglS∆102 bglT/bglS∆EV aprE nprE his BGSC
Kno6 eglS∆102 bglT/bglS∆EV aprE nprE hisbpreprHtrAmprnprBvpr This work
1A751C 1A751 containing pMATE; Kmr This work
1A751F1 1A751 containing pMATEF; Kmr This work
1A751F2 1A751 containing pMA5F; Kmr This work
CIS1 1A751 containing pMATEFc1; Kmr This work
CIS2 1A751 containing pMATEFc2; Kmr This work
CIS3 1A751 containing pMATEFc3; Kmr This work
CIS4 1A751 containing pMATEFc4; Kmr This work
CIS5 1A751 containing pMATEFc5; Kmr This work
CIS6 1A751 containing pMATEFc6; Kmr This work
CIS7 1A751 containing pMATEFc7; Kmr This work
CHAP1 CIS5 containing pDF1; Cmr, Kmr This work
CHAP 2 CIS5 containing pDF2; Cmr, Kmr This work
CHAP 3 CIS5 containing pDF3; Cmr, Kmr This work
CHAP 4 CIS5 containing pDF4; Cmr, Kmr This work
CHAP 5 CIS5 containing pDF5; Cmr, Kmr This work
CHAP 6 CIS5 containing pDF6; Cmr, Kmr This work
CHAP 7 CIS5 containing pDF7; Cmr, Kmr This work
CHAP 8 CIS5 containing pDF8; Cmr, Kmr This work
CHAP 9 CIS5 containing pDF9; Cmr, Kmr This work
CHAP 10 CIS5 containing pDF10; Cmr, Kmr This work
CHAP 11 CIS5 containing pDF11; Cmr, Kmr This work
CHAP3R1 CHAP3 containing pMATEFc5R1; Cmr, Kmr This work
CHAP3R2 CHAP3 containing pMATEFc5R2; Cmr, Kmr This work
CHAP3R3 CHAP3 containing pMATEFc5R3; Cmr, Kmr This work
Kno6cs Kno6 containing pMATEFc5; Kmr This work
Kno6cf Kno6 containing pDF3, pMATEFc5; Cmr, Kmr This work
Kno6k Kno6 containing pDF3; Cmr This work
Kno6csp 1 Kno6k containing pMATEF1; Cmr, Kmr This work
Kno6csp 2 Kno6k containing pMATEF2; Cmr, Kmr This work
Kno6csp 3 Kno6k containing pMATEF3; Cmr, Kmr This work
Kno6csp 4 Kno6k containing pMATEF4; Cmr, Kmr This work
Kno6csp 5 Kno6k containing pMATEF5; Cmr, Kmr This work
Kno6csp 6 Kno6k containing pMATEF6; Cmr, Kmr This work
Kno6csp 7 Kno6k containing pMATEF7; Cmr, Kmr This work
Kno6csp 8 Kno6k containing pMATEF8; Cmr, Kmr This work
Kno6csp 9 Kno6k containing pMATEF9; Cmr, Kmr This work
Kno6csp 10 Kno6k containing pMATEF10; Cmr, Kmr This work
Kno6csp 11 Kno6k containing pMATEF11; Cmr, Kmr This work