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Fig. 6 | Microbial Cell Factories

Fig. 6

From: High-efficiency expression and secretion of human FGF21 in Bacillus subtilis by intercalation of a mini-cistron cassette and combinatorial optimization of cell regulatory components

Fig. 6

Comparison of efficiency of different signal peptides. a SDS-PAGE analysis of different signal peptides in culture medium following incubation for 24 h. The molecular weight of mature rhFGF21 is about 27 kDa. Column M: molecular weight marker. Column Ev: plasmids of pMATE of strain 1A751C served as negative control. b The relative densitometric analysis for rhFGF21 secreted by different B. subtilis strains. c Western blotting analysis of the secreted rhFGF21 protein with different signal peptides in B. subtilis

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