Fig. 5From: High-efficiency expression and secretion of human FGF21 in Bacillus subtilis by intercalation of a mini-cistron cassette and combinatorial optimization of cell regulatory componentsSDS-PAGE analysis of expression of six extracellular proteases deficient strain Kno6cf in culture medium following 24 h incubation. The molecular weight of mature rhFGF21 is about 25 kDa. Column M: molecular weight marker. The strain 1A751C served as negative controlBack to article page