Fig. 2

Transcriptional landscape in the WT during the shift from glucose to a condition of interest. Double hierarchical clustering of normalized fold change (FC) values, calculated using glucose as the calibrator sample, and expressed as log₂ (FC). Clustered GOIs (top dendogram) and culture conditions (left dendogram), with the corresponding names at the bottom and right of the heatmap, respectively. The white lines in the heat map delineate the main clusters, i.e. 5 and 4 clusters for GOIs and culture conditions, respectively. The coloured bar at the top of the heat map is referring to functional categories affiliation of GOIs (legend at the bottom left of the figure). Red and green colours indicate increased and decreased FC values, respectively (scale at the bottom of the figure, with saturating intensity for absolute values of log₂ (FC) beyond 9). Cq values that exceeded 35 cycles were considered beyond the limit of detection and represented by the grey cases in the heat map. The bottom, light blue heat map depicts the number of each of the 3 binding motifs identified in the 700 bp upstream the ATG start codon of each gene, i.e. the XlnR binding motif -5′-GGCTAA-3′, its low affinity variant 5′-GGCWAW-3′, and the CreA binding motif -5′-SCGGRG-3′