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Table 1 Bacterial strains and plasmids used in this study

From: Neutralizing-antibody-mediated protection of chickens against infectious bursal disease via one-time vaccination with inactivated recombinant Lactococcus lactis expressing a fusion protein constructed from the RCK protein of Salmonella enterica and VP2 of infectious bursal disease virus

Strain or plasmid

Relevant characteristics

Source/reference

Strains

 NZ3900

Host for food-grade use of the NICE system by plasmids election based on the ability to grow on lactose as carbon source.

MoBiTec

 L. lactis

L. lactis NZ3900 containing pNZ8149 plasmid

MoBiTec

 L. lactis-RCK

L. lactis NZ3900 containing pNZ8149-RCK plasmid

This study

 L. lactis-VP2-RCK

L. lactis NZ3900 containing pNZ8149-VP2-RCK

This study

Plasmid

 pNZ8149

High-copy number lactococcal vector

MoBiTec

 pNZ8149-RCK

Vector carrying rck gene

This study

 pNZ8149-VP2-RCK

Vector carrying VP2 gene and rck gene

This study