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Table 1 Bacterial strains and plasmids used in this study

From: Neutralizing-antibody-mediated protection of chickens against infectious bursal disease via one-time vaccination with inactivated recombinant Lactococcus lactis expressing a fusion protein constructed from the RCK protein of Salmonella enterica and VP2 of infectious bursal disease virus

Strain or plasmid Relevant characteristics Source/reference
Strains
 NZ3900 Host for food-grade use of the NICE system by plasmids election based on the ability to grow on lactose as carbon source. MoBiTec
 L. lactis L. lactis NZ3900 containing pNZ8149 plasmid MoBiTec
 L. lactis-RCK L. lactis NZ3900 containing pNZ8149-RCK plasmid This study
 L. lactis-VP2-RCK L. lactis NZ3900 containing pNZ8149-VP2-RCK This study
Plasmid
 pNZ8149 High-copy number lactococcal vector MoBiTec
 pNZ8149-RCK Vector carrying rck gene This study
 pNZ8149-VP2-RCK Vector carrying VP2 gene and rck gene This study