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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Neutralizing-antibody-mediated protection of chickens against infectious bursal disease via one-time vaccination with inactivated recombinant Lactococcus lactis expressing a fusion protein constructed from the RCK protein of Salmonella enterica and VP2 of infectious bursal disease virus

Fig. 1

Construction of the plasmid pNZ8149-OptiVP2-RCK expressing the VP2-RCK fusion protein. a The recombination plasmid pNZ8149-OptiVP2-RCK was linearized with NcoI to obtain a 3975-bp fragment (lane 2). The recombinant plasmid pNZ8149-OptiVP2-RCK was identified via digestion with NcoI and KpnI to obtain a 2646-bp fragment and a 1329-bp fragment (lane 3). The molecular mass standard sizes of the DNA marker are indicated to the left. b PCR amplification of Opti-VP2 optimization gene (1329 bp). c schematic diagrams of the recombinant plasmid pNZ8149-OptiVP2-RCK with the VP2-RCK gene fusion

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