Recombinant O-mannosylated protein production (PstS-1) from Mycobacterium tuberculosis in Pichia pastoris (Komagataella phaffii) as a tool to study tuberculosis infection

Table 4 Theoretical and obtained peptides mass generated by Trypsin digestion of rPstS-1 produced in P. pastoris

Peptide name	Position of cleavage site	Peptide	Length (aa)	Theoretical Peptide mass (Da)	Modification	Peptide mass (Da)
P1T	54	AGCGSKPPSGSPETGAGAGTVATTPASSPVTLA ETGSTLLYPLFNLWGPAFHER	54	5315.900		ND
P2T	97	YPQVTITAQGTGSGAGIAQAAAGTVNIGASDAYL SEGDMAAHK	43	4122.489		B) 4120.98
P3T	123	GLMNIALAISAQQVNYNLPGVSEHLK	26	2781.222 +2 Hex 3105.22 +4 Hex 3429.22 +5 Hex 3591.22	P3T + 2 Hex P3T + 4 Hex P3T + 5 Hex	B) 3105.78 C) 3429.46 C) 3590.15
P4T	127	LNGK	4	430.504		ND
P5T	138	VLAAMYQGTIK	11	1194.455	P5T + 6 Hex	B) 2166.65
P6T	164	TWDDPQIAALNPGVNLPGTAVVPLHR	26	2752.125		B) 2751.45
P7T	180	SDGSGDTFLFTQYLSK	16	1765.894		C) 1765.82
P8T	188	QDPEGWGK	8	915.958		ND
P9T	238	SPGFGTTVDFPAVPGALGENGNGGMVTGCAE TPGCVAYIGISFLDQASQR	50	4918.459		C) 4916.29
P10T	270	GLGEAQLGQSSGNFLLPDAQSIQAAAAGFASK	32	3105.411		B) 3104.56 C) 3105.89
P11T	301	TPANQAISMIDGPAPDGYPIINYEYAIVNNR	31	3378.761		B) 3377.64 C) 3379.80
P12T	303	QK	2	274.320		ND
P13T	324	DAATAQTLQAFLHWAITDGNK	21	2272.502		B) 2272.13 C) 2271.81
P14T	342	ASFLDQVHFQPLPPAVVK	18	1993.336		B) 1993.08 C) 1994.49
P15T	353	LSDALIATISS	11	1090.239	P15T + 4 Hex	B) 1738.59

ISSN: 1475-2859