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Table 1 Stoichiometric and kinetic parameters of P. pastoris growth and rPstS-1 production cultured in baffled shake flasks and in 1.0 L bioreactors

From: Recombinant O-mannosylated protein production (PstS-1) from Mycobacterium tuberculosis in Pichia pastoris (Komagataella phaffii) as a tool to study tuberculosis infection

Parameter Baffled shake flask Bioreactor
Max O.D. 600 nm glycerol (A.U.) 26.5 ± 0.4 31.5 ± 2.2b
Max O.D. 600 nm methanol (A.U.) 32.7 ± 0.8 47.0 ± 2.6a
Max biomass glycerol (g/L) 8.8 ± 0.2 g/L 10.4 ± 0.9b
Max biomass methanol (g/L) 10.8 ± 0.3 g/L 15.5 ± 0.9a
μ glycerol (/h) 0.323 ± 0.025 0.286 ± 0.033
μ methanol (/h) 0.003 ± 0.001 0.006 ± 0.001b
Yx/s (gDCW/gGlyc) 0.69 ± 0.01 0.81 ± 0.03b
Yx/s (gDCW/gMeth) 0.051 ± 0.004 0.092 ± 0.004a
qs (gGlyc/gDCW h) 0.021 ± 0.004 0.034 ± 0.013
Supernatant total protein (mg/L) 127 ± 15 185 ± 9a
YSTP/DCW (mg STP/g DCW) 11.75 ± 1.13 11.92 ± 0.59
rPstS-1 (mg/L) 46 ± 5 46 ± 4
qrPstS-1 (mg/L h) 0.639 ± 0.070 0.633 ± 0.054
qp(rPstS-1) (mgrPstS-1/gDCW h) 0.059 ± 0.002 0.040 ± 0.004a
YrPstS-1/x (mgrPstS-1/gDCW) 3.3 ± 0.01 4.0 ± 0.02a
  1. The mean and standard deviation for at least three biological replicates are shown
  2. O.D., optical density; A.U., absorbance units; µ, specific growth rate; STP, supernatant total protein; DWC, dry cell weight; Yx/s, total dry cell weight yield per glycerol; Yx/s, total dry cell weight yield per methanol; YSTP/DCW, STP yield per DCW; qs, specific consumption rate of glycerol per DCW; qPstS-1, volumetric productivity of rPstS-1; qp(rPstS-1), specific productivity of rPstS-1; YrPstS-1/x, rPstS-1 yield per DCW. µ was calculated in first 8 h of culture
  3. p value was calculated using t test by comparing the results of Shake flask VS Bioreactor
  4. ap < 0.005; b p < 0.05