Fig. 5

MALDI-TOF spectra of HPLC purified rPstS-1 antigen. a Non-digested samples were placed on a CHCA (α-Cyano-4-hydroxycinnamic acid) matrix and analyzed in a matrix assisted laser desorption ionization time-of-flight (MALDI-TOF, Bruker Microflex) equipment with a 20-Hz nitrogen laser at I = 337 nm. Spectra was recorded in linear positive mode for the mass range 20,000 to 100,000 Da. b MALDI-TOF–MS analysis (linear mode) of a tryptic digest of rPstS-1, sample mixed with α-cyano-4-hydroxycinnamic acid matrix. c MALDI-TOF–MS analysis (linear mode) of a tryptic digest of rPstS-1, sample mixed with α-cyano-4-hydroxycinnamic acid and 3,5-dimethoxy-4-hydroxycinnamic acid matrices. d MALDI-TOF–MS analysis (linear mode) of a Lys-C digest of rPstS-1. Tryptic and Lys-C peptides marked denote mass peaks corresponding to predicted peptides shown in Tables 4 and 5, respectively. Mannose residues were detected with differences to theoretical and experimental data of 162 Da mark as Hex. All detections are presented in Table 4 and 5