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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Recombinant O-mannosylated protein production (PstS-1) from Mycobacterium tuberculosis in Pichia pastoris (Komagataella phaffii) as a tool to study tuberculosis infection

Fig. 2

Total protein analysis from culture supernatant. a SDS-PAGE 12% of protein obtained from baffled shake flask cultures, b SDS-PAGE 12% of protein obtained from bioreactor supernatants. Black arrows indicate the band corresponding to rPstS-1. c Immunodetection of rPstS-1 from bioreactor supernatants with the polyclonal antibody anti-PstS1, black arrows indicate the immunodetected recombinant protein. The post-induction time for each sample taken is indicated at the top of each lane. M, molecular weight; C−, negative control; supernatant sample from a wild type P. pastoris X-33 culture

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Microbial Cell Factories

ISSN: 1475-2859

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