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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Protein production in Escherichia coli is guided by the trade-off between intracellular substrate availability and energy cost

Fig. 2

a Distribution of elongation step duration (tenc,s, bars) in the reaction volume and the resulting overall elongation time (sum of elongation step duration, black line) for the first 3000 elongation steps of GFP as the target sequence (in vivo conditions). b Evaluation of elongation step duration in vivo and in vitro for GFP, codon optimized GFP (GFP*), and EFTu as the target sequence

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