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Fig. 9 | Microbial Cell Factories

Fig. 9

From: Rational construction of genome-reduced and high-efficient industrial Streptomyces chassis based on multiple comparative genomic approaches

Fig. 9

Morphological differentiation, actinorhodin production and genetic stability analyses. a Mycelia of L104 formed aggregated hyphae or noticeable pellets on glass wall, but L324 mutant cannot. (B) Mycelia were observed by microscope at magnifications 10×, 20×, and 40×. L104 and L324 were inoculated in YEME medium about 120 h. c The actinorhodin production in L104 and L324 were measured at different times. d Genetic stability of L104 and L324. Strains are performed serial passaging on YMG plate and actinorhodin production in YMG plate was determined after 10 days incubation. The error bars represent standard deviations of the means of triplicate samples. e LC–MS analysis of actinorhodin in negative ion mode from metabolites of L104 and L324

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