Table 1 Strains and plasmids
Strains and plasmids | Relevant characteristics | Source |
|---|---|---|
Strains | ||
E. coli MG1655 | Wild type | Laboratory collection |
Clostridium acetobulylicum ATCC 184 | Wild type | ATCC |
JW3366-1 | BW25113, ΔpckA::KmR | Coli genetic stock center (CGSC) |
JW2447-5 | BW25113, ΔmeaB::KmR | Coli genetic stock center (CGSC) |
JW1122-2 | BW25113, ΔicdA::KmR | Coli genetic stock center (CGSC) |
HY01 | MG1655, Ptrc-ackA-pta | This study |
HY021 | MG1655, Ptrc-ackA-pta, ΔpckA | This study |
HY022 | MG1655, Ptrc-ackA-pta, ΔmaeB | This study |
HY031 | MG1655, Ptrc-ackA-pta, ΔpckA, ΔmaeB | This study |
HY041 | MG1655, Ptrc-ackA-pta, ΔpckA, ΔmaeB, ΔicdA | This study |
Plasmids | ||
pKD4 | oriR6Kγ, KmR, rgnB (Ter) | Datsenko and Wanner [33] |
pKD46 | araBp-gam-bet-exo, bla (ApR), repA101 (ts), oriR101 | Datsenko and Wanner [33] |
pCP20 | ApR, CmR, FLP recombinance | Datsenko and Wanner [33] |
pTrc99a | Cloning vector, trc promotor, ApR | Laboratory collection |
pTrc99a-trc-atoDA | pTrc99a carries trc promoter with atoD and atoA from E. coli | This study |
pTrc99a-trc-ctfAB | pTrc99a carries trc promoter with ctfA and ctfB from Clostridium acetobulylicum | This study |
pTrcBAD | pTrc99a-trc-atoDA carries atoB from E. coli and adc from Clostridium acetobulylicum | This study |
pTrcTAD | pTrc99a-trc-atoDA carries thl and adc from Clostridium acetobulylicum | This study |
pTrcBAC | pTrc99a-trc-ctfAB carries atoB from E. coli and adc from Clostridium acetobulylicum | This study |
pTrcTAC | pTrc99a-trc-ctfAB carries thl and adc from Clostridium acetobulylicum | This study |