Table 1 Soluble and pure scFv protein yields recovered by mild solubilization and in vitro refolding methods
| Â | Methods | Solublilized IB protein (mg) | Solubility and refolding yield (mg) | Solubility and refolding yield (%) | Soluble and pure protein yield (mg) | Recovery yield of pure scFv (%) |
|---|---|---|---|---|---|---|
scFv | Mild solubilization | 42.67 ± 6.0 | 19.84 ± 4.8 | 46.09 ± 4.3 | 12.3 ± 2.7 | 29.15 ± 1.8 |
His-scFv | Denaturation–refolding | 65.33 ± 5.1 | 7.24 ± 1.3 | 11.08 ± 1.7 | 4.73 ± 0.5 | 7.26 ± 0.6 |
GST-scFv | Denaturation–refolding | 72.0 ± 4.2 | 5.13 ± 0.8 | 7.12 ± 0.7 | 1.18 ± 0.4 | 1.67 ± 0.65 |
MBP-scFv | Denaturation–refolding | 35.33 ± 5.4 | 17.42 ± 3.7 | 49.3 ± 4.8 | 15.59 × 3.4 | 44.13 ± 4.7 |