Fig. 3

CD spectrum of scFv antibody protein with and without different fusion tags were recorded and analyzed with CAPITO. The graphical output for its area difference method is also shown. The best matching of reference dataset are scFv without any tag (orange) recovered by mild-solubilization method while scFvs achieved by denaturation–refolding method with different tags have not hit with reference data at all. Lower panel: the CD values at λ = 200 nm was plotted versus values λ = 222 nm to deduce the folding state of scFv in presence and absence of different tags