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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Evaluation of scFv protein recovery from E. coli by in vitro refolding and mild solubilization process

Fig. 2

FPLC chromatogram and SDS-PAGE of purified scFv proteins without and with different fusion tags. a The chromatogram for tag free scFv protein solution achieved by using mild solubilizing agents and the pure scFv peak fraction was separated on SDS-PAGE. The chromatograms of b His-scFv, c GST-scFv and d MBP-scFv protein solutions achieved by complete denaturation followed by refolding-dialysis method and the pure peak fractions were also separated on SDS-PAGE

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Microbial Cell Factories

ISSN: 1475-2859

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