Table 1 List of kcat values and protein quantifications integrated in iYO844 model
Reaction name | Gene name | EC | Equation | kcat [s−1] | [E] [mmol/gDW] | Organism of kcat data | Refs. for kcat data |
|---|---|---|---|---|---|---|---|
PGI | pgi | 5.3.1.9 | g6p → f6p | 126 | 1.55 × 10−5 | E. coli | [59] |
TPI | tpiA | 4.1.1.31 | dhap → g3p | 150 | 1.28 × 10−5 | E. coli | [60] |
GAPD_NAD | gapA | 1.2.1.12 | g3p + nad +pi → 13dpg + h + nadh | 70 | 5.77 × 10−5 | B. subtilis | [61] |
PGK | pgk | 2.7.2.3 | 13dpg + adp → 3pg + atp | 329 | 3.60 × 10−5 | E. coli | [62] |
PGM | pgm | 5.4.2.12 | 3pg → 2pg | 765.9 | 8.85 × 10−6 | B. subtilis | [63] |
ENO | eno | 4.2.1.11 | 2pg → h2o + pep | 130.4 | 3.17 × 10−5 | B. subtilis | [64] |
G6PDH | zwf | 1.1.1.49 | g6p + nadp → 6pgl + h + nadph | 174 | 8.05 × 10−6 | E. coli | [65] |
CS | citZ | 2.3.3.16 | accoa + h2o + oaa → cit + coa + h | 49 | 2.51 × 10−5 | B. subtilis | [30] |
ICDHy | icd | 1.1.1.42 | icit + nadp → akg + co2 + nadph | 82 | 1.10 × 10−4 | B. subtilis | [66] |
FUM | citG | 4.2.1.2 | fum + h2o → mal-l | 283.3 | 7.29 × 10−6 | E. coli | [67] |
MDH | mdh | 1.1.1.37 | mal-l + nad → h + nadh + oaa | 177.1 | 1.06 × 10−4 | B. subtilis | [68] |
PTAr | pta | 2.3.1.8 | accoa + pi → actp + coa | 651.6 | 8.49 × 10−6 | B. subtilis | [69] |
LDH_L | ldh | 1.1.1.27 | lac-l + nad → h + nadh + pyr | 6416.6 | 3.60 × 10−6 | B. subtilis | [70] |
PGCDr | serA | 1.1.1.95 | 3pg + nad → 3php + h + nadh | 14.56 | 1.90 × 10−5 | B. subtilis | [71] |
OXADC | oxdC | 4.1.1.2 | h + oxa → co2 + for | 59 | 6.21 × 10−7 | B. subtilis | [72] |
MICITL | yqiQ | 4.1.3.30 | micit → pyr + succ | 19 | 6.80 × 10−8 | E. coli | [73] |
OXGDC | menD | 4.1.1.71 | akg +h → co2 + sucsal | 0.2 | 6.80 × 10−8 | B. subtilis | [55] |