Fig. 3

Isoprene production of strains modified with weakened RBS sequence of non-key enzymes, ERG19 and MvaE. a Illustration of the constructed plasmids in this study and predicted T.I.R. of every gene. The height of the red columns indicated the T.I.R. value. b Isoprene production of strain with modification of RBS sequence of MvaE. c Isoprene production of strain with modification of RBS sequence of ERG19. The black column indicated the isoprene production of the control strain. The gray column indicated the isoprene production of strain with RBS sequence optimization. The dot indicated the corresponding T.I.R. values. The experiment was conducted in triplicate. Bar represents mean ± s.d