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Table 2 Kinetic data of wild-type and Q155V/C158G CeGNA1 for GlcN-6-P

From: Combinatorial pathway enzyme engineering and host engineering overcomes pyruvate overflow and enhances overproduction of N-acetylglucosamine in Bacillus subtilis

  Km (μM) kcat (s−1) kcat/Km (s−1 μM−1)
CeGNA1 139 ± 9 136 ± 1.1 0.98
CeGNA1-Q155V/C158G 122 ± 6 151 ± 1.5 1.23