From: Combinatorial pathway enzyme engineering and host engineering overcomes pyruvate overflow and enhances overproduction of N-acetylglucosamine in Bacillus subtilis
Km (μM)
kcat (s−1)
kcat/Km (s−1 μM−1)
CeGNA1
139 ± 9
136 ± 1.1
0.98
CeGNA1-Q155V/C158G
122 ± 6
151 ± 1.5
1.23