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Table 2 Kinetic data of wild-type and Q155V/C158G CeGNA1 for GlcN-6-P

From: Combinatorial pathway enzyme engineering and host engineering overcomes pyruvate overflow and enhances overproduction of N-acetylglucosamine in Bacillus subtilis

 

Km (μM)

kcat (s−1)

kcat/Km (s−1 μM−1)

CeGNA1

139 ± 9

136 ± 1.1

0.98

CeGNA1-Q155V/C158G

122 ± 6

151 ± 1.5

1.23