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Table 1 Strains and plasmids used in this study

From: Combinatorial pathway enzyme engineering and host engineering overcomes pyruvate overflow and enhances overproduction of N-acetylglucosamine in Bacillus subtilis

  Characteristics References
Strains
BSGN5 B. subtilis 168 derivate, ΔnagPΔgamPΔgamAΔnagAΔnagBΔldh::lox72 [30]
BSGN10 B. subtilis 168 derivate, ΔnagPΔgamPΔgamAΔnagAΔnagBΔldhΔalsRSD::lox72 [5]
BSGN12 B. subtilis 168 derivate, ΔnagPΔgamPΔgamAΔnagAΔnagBΔldhΔptaΔackA::lox72, ΔalsRSD::Pveg-ΔermC+14/7A-ecglmS [6]
BSGN12-Pveg-urease BSGN12 derivate, expression of urease from Bacillus paralicheniformis under the control of promoter Pveg This study
BSGN12-PxylA-urease BSGN12 derivate, expression of urease from Bacillus paralicheniformis under the control of promoter PxylA This study
BSGN12-PabrB-urease BSGN12 derivate, expression of urease from Bacillus paralicheniformis under the control of promoter PabrB This study
BSGN13 BSGN12 derivate, expression of urease from Bacillus paralicheniformis under the control of promoter Phag This study
BSGN12-Pffh-urease BSGN12 derivate, expression of urease from Bacillus paralicheniformis under the control of promoter Pffh This study
BSGN12-PlicH-urease BSGN12 derivate, expression of urease from Bacillus paralicheniformis under the control of promoter PlicH This study
Plasmids   This study
p7Z6 pMD18-T containing lox71-zeo-lox66 cassette [19]
pTSC EmrAmpr; temperature sensitive in B. subtilis [19]
pP43-cMyc (M-Rm)-CeGNA1 key enzyme CeGNA1 expressing vector [6]
pP43-cMyc (M-Rm)-CeGNA1-Q155V/C158G pP43-cMyc (M-Rm)-CeGNA1 derivate, with 155Gln and 158Cys of CeGNA1 mutated to 155Val and 158Gly, respectively This study
pP43-6His-CeGNA1 pP43NMK derivate with CeGNA1 cloned [6]
pCold-CeGNA1 pCold III derivate, containing wild type CeGNA1, with His tag fused to the N terminal This study
pCold-CeGNA1-Q155V/C158G pCold III derivate, containing mutant CeGNA1-Q155V/C158G, with His tag fused to the N terminal This study
pPvegEcGlmS-1 pUC57-Amp derivate, containing the synthesized expression cassette, terminator I-Pveg-TSS-RBS0-terminator II [6]
pStop1622 Ampr, Tetr, E. coli-B. megaterium shuttle vector [31]