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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Combinatorial pathway enzyme engineering and host engineering overcomes pyruvate overflow and enhances overproduction of N-acetylglucosamine in Bacillus subtilis

Fig. 5

Time profile of fed-batch fermentation of BSGN13 in a 3-L fermenter. In the fed-batch fermentation, inoculation size, temperature, pH, agitation speed, and aeration rate were 5%, 37 °C, 7.3, 800 rpm, and 1.5 vvm, respectively. With the initial concentration being 40 g/L, glucose concentration was maintained at 3–10 g/L using the automatic glucose analyzer during the fermentation. DCW: dry cell weight

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